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Defender Ryan Bertrand is a doubt for England friendly against Portugal at Wembley on Thursday, their final warm-up game before Euro 2016.
The Southampton full-back is suffering from a knock which kept him out the first training session since manager Roy Hodgson named his 23-man squad.
He is set to be fit for the tournament in France, which starts on 10 June.
Midfielders Jordan Henderson and Jack Wilshere, who have had fitness concerns, trained on Wednesday.
England play Russia in their opening Group B game on 11 June, Wales on 16 June and Slovakia on 20 June.
A sneak peek at Hodgson’s XI?
England played two friendlies in May, beating Turkey and Australia 2-1 as Hodgson experimented with his line-up.
Against Turkey, Hodgson used a 4-4-2 formation with a midfield diamond, while he opted for a 4-3-3 against Australia.
Manchester United’s 18-year-old forward Marcus Rashford scored on his England debut against the Socceroos.
England travel to their Euro 2016 base in Chantilly on Monday.
No Ronaldo, no worries for Portugal
Cristiano Ronaldo will not play for Portugal against England, having been granted time off after scoring the winning spot-kick in Real Madrid’s penalty shootout win over Atletico Madrid in Saturday’s Champions League final.
Portugal beat Norway in a friendly in Porto on Sunday without Ronaldo, and coach Fernando Santos has expressed his frustration at being repeatedly asked about the forward.
“I don’t understand why we always have to talk about Ronaldo,” said Santos.
“I’m not in the least bit worried about him. He’ll be here next week with the goal of helping Portugal have a great Euro 2016. The rest is just journalist talk.”
Portugal start their Euro 2016 campaign against Iceland on 14 June. Austria and Hungary also feature in Group F.